Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. This starter kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, GlycoWorks Sample Collection Module, as well as the ACQUITY Premier UPLC Glycan BEH Amide, 1.7 µm, 2.1 x 150 Column and Mobile-Phase Concentrate: Ammonium Formate. It contains materials to process 24 samples in a 3x8 format. The kit contains: GlycoWorks Deglycosylation Module: (1) 0.035 ml/vial Rapid PNGaseF Enzyme (1) 0.25 ml/vial Glycoworks Rapid Buffer (3) 4 vials of 10 mg/vial RapiGest SF Surfactant (1) Intact mAb Mass Check Standard *Shipped in a separate box GlycoWorks RapiFluor-MS Labeling Module: (3) 9 mg/vial RapiFluor-MS Reagent (3) 1 ml/vial Reagent Solvent (Anhydrous DMF) GlycoWorks RFMS Clean-up Module: (4) 5 ml/vial Elution Buffer (200 mM Ammonium Acetate 95/5 Water/ACN pH 7) (4) 3 ml/vial Sample Diluent (DMF/ACN Mixture) (1) GlycoWorks HILIC µElution Plate GlycoWorks Sample Collection Module: (15) 1 mL tubes for deglycosylation and labeling reaction (15) 600 uL tube inserts for SPE eluate collection (15) Cap strips with slits for direct injection (1) 1 mL round collection tray (1) Waste tray Extras: (1) 5050 mM Ammonium Formate Solution—Glycan Analysis (186007081) (1) ACQUITY Premier UPLC Glycan BEH Amide Column, 130 Å, 1.7 µm, 2.1 x 150 mm (186009549) (1) RapiFluor-MS Glycan Performance Test Standard
The Deglycosylation Module and standards will ship cold chain. All other modules will ship at room temperature.
